Phylogenetic analysis of some fungi species in West Kazakhstan based on nuclear ribosomal DNA ITS sequences

Authors

  • Emre SEVİNDİK Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar (TR)
  • Assemgul N. SARSENOVA L.N. Gumilyov Eurasian National University, Department of General Biology and Genomics, Satpayev str. 2, Nur-Sultan (KZ)
  • Abiev S. ABIEVICH L.N. Gumilyov Eurasian National University, Department of General Biology and Genomics, Satpayev str. 2, Nur-Sultan (KZ)
  • Erengül SOFYALIOĞLU Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar (TR)
  • Darbayeva T. ESENOMANOVNA Makhambet Utemisov West Kazakhstan University, N. Nazarbayev ave.162, Uralsk (KZ)
  • Cafer EKEN Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar, Aydin (TR)

DOI:

https://doi.org/10.15835/nbha50312783

Keywords:

fungi, phylogenetic analysis, ITS, West Kazakhstan

Abstract

In this study, phylogenetic analysis of some fungal species distributed in West Kazakhstan was performed based on ITS sequences. All of the mushroom samples were collected from different regions of West Kazakhstan and brought to the laboratory. Total genomic DNA was extracted using a GeneMark commercial kit. ITS1 and ITS4 primers were used for the amplification of the ITS region in PCR analyses. The resulting DNA sequences were then edited using BioEdit and FinchTV. For phylogenetic analysis used MEGA 6.0 program. As a result of the study, ITS sequences ranged from 532 to 715 nucleotides, while the divergence values of the sequences differed between 0.000 and 0.468. The maximum likelihood tree constructed using ITS sequences consists of two clades. According to the phylogenetic analysis results obtained using other fungal species, ITS results were found to be a good indicator for the differentiation of fungal genera.

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Published

2022-09-12

How to Cite

SEVİNDİK, E., SARSENOVA, A. N., ABIEVICH, A. S., SOFYALIOĞLU, E., ESENOMANOVNA, D. T., & EKEN, C. (2022). Phylogenetic analysis of some fungi species in West Kazakhstan based on nuclear ribosomal DNA ITS sequences. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12783. https://doi.org/10.15835/nbha50312783

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Section

Research Articles
CITATION
DOI: 10.15835/nbha50312783